High-performance Liquid Chromatograph

Item No.: 4-0-0-3

This high performance liquid chromatograph uses chromatographic separation techniques and detection techniques to detect organic compounds that are high in boiling, non-volatile, thermally unstable, and macromolecules. It is a common instrument for precision analysis and testing in laboratories.

INQUIRY

Description

Description: This high performance liquid chromatograph uses chromatographic separation techniques and detection techniques to detect organic compounds that are high in boiling, non-volatile, thermally unstable, and macromolecules. The system is mainly used in food safety, drug analysis, environmental protection and health and epidemic prevention, agriculture, forestry, fisheries, animal husbandry, manufacturing, petrochemical, quality supervision, teaching research, water conservancy systems and other fields. It is a common instrument for precision analysis and testing in laboratories.

Working Principle of High-performance Liquid Chromatograph
As shown on the right: High performance liquid chromatograph 2

    A — Solvent (Mobile phase, flowing carrier)
    B — High-pressure pump
    C — Pressure gauge
    D — Sample, liquid (The sample is "injected" into the system by a microsyringe)
    E — Sample mixer
    F — HPLC column
    G — Detector
    H — Data processor and computer workstation
    I — Waste liquid, recycled by container
The solvent (A) in the bottle, hereinafter referred to as the mobile phase, is pumped into the detection system by the high pressure pump (B). The sample (D, liquid) is "injected" into the sample mixer (E) by a microsyringe and mixed with the mobile phase, is loaded into the HPLC separation column (F) by the mobile phase. There are some fixed particles in the HPLC separation column, hereinafter referred to as the stationary phase. Since the components in the sample have different partition coefficients in the two phases (mobile phase and stationary phase), when they are relatively moved in two phases, after repeated "adsorption-desorption" distribution, the components have a large difference in the moving speed, they are separated into individual components and flow out of the column in sequence. When thes

e components pass through the detector (G), its concentration is converted into an electrical signal. These electrical signals are processed by the data processor and computer workstation (H) and displayed as a peak shape map. The imagen of HPLC peak shape as shown on the right.
According to the separation method, HPLC can be divided into liquid-solid adsorption chromatography, liquid-liquid partition chromatography (normal phase and reverse phase), ion exchange chromatography and size exclusion chromatography.

Performance and parameters of LC-31 high performance liquid chromatograph
◇High-pressure Pump
The reciprocating double-plunger parallel pump with intelligent control of the microprocessor has the characteristics of high pressure

resistance, small pulsation, stable and reliable, and convenient operation. Due to the alternating infusion of the dual pump heads, the piston rod and seal life is twice as long as the conventional tandem pump in the same infusion volume. The mechanical components of the pump are all computer-aided design with high machining accuracy. Each pump undergoes a rigorous pressure test so that the pump is leak free. The high pressure pump has a condition monitoring function. The microprocessor controls the flow and pressure of the pump in real time. When the pressure exceeds the limit value, an alarm is issued and the pump is automatically stopped.
Technical Parameters of High Pressure Pump
•Flow rate Control Range: 0.001～9.999mL/min(step adjustable flow rate:0.001mL/min)
•Flow rate stability deviation: Sr≤0.3％ RSD<0.006％
•Specification Error SS: SS≤2％(Flow rate 1mL/min, pure water, pressure 5～10Mpa, room temperature)
•Pressure linearity and test accuracy: indicates pressure error smaller than 0.5Mpa (0～42MPa)
•Pulse pressure:≤0.1MPa (Flow rate 1mL/min, pressure 5～10Mpa)
•Airproof of pump: pressure 42MPa, time 10min, and pressure fall< 0.5MPa
•Maximal operating pressure: 42MPa(flow rate: 0.001～9.999mL/min)
•Dimension: 450mmX300mmX160mm (length x width x height)
◇UV Detector
With the ground breaking digital switch system, the detector directly transfers the digital signals to WS1000 work station, eliminating the High performance liquid chromatograph 5

distortion and interference caused by multiple analog-to-digit conversions of chromatographic signals of common UV detector.
The outstanding flow cell design significantly improves the technical indexes of the instrument. The optical system adopts the precise positioning structure with high accuracy and tiny deviation. As the heat insulation technology is applied between D2 lamp and optical system, the period for stabilization is shortened and the influence the D2 lamp has on the optical path minimized.
The preamplifier combines a high resolution A/D switch with a high resistant and low drifting instrument amplifier. In routine analyses, the dynamic range amounts to 106, ensuring the accuracy of the logarithmic calculation.
Technical Parameters of UV detector
•Wavelength range: 190－680nm
•Spectral bandwidth: 8nm
•Wavelength indicated value error: ≤±1nm
•Wavelength repetitiveness: better than 0.1nm
•Baseline noise: ≤2X10－5AU(Dynamic)
•Baseline drift: ≤2X10－4AU(Dynamic)
•Minimum detectable concentration: 1 X 10－8g/ mL (naphthalene/methanol solution)
•Dimension: 450mmX300mmX160mm (length x width x height)

◇High performance high pressure check valve High performance liquid chromatograph 6

The valve body, the diamond ball seat and the diamond ball are integrated into a single unit, featuring simple structure and good sealing capability. The unit enhances flow rate accuracy of the pump and measurement precision of the system. It is easy to install and replace.

◇Design of Floating Guider
The floating guide mechanism extends the lifespan of the piston collar and delivers solvents stably in longer term.

◇Post-column Rinsing Pump

If buffering salt acts as the mobile phase, it will accumulate at post column and cause mechanical wearing when not rinsed on time. Post-column Rinsing Pump is the answer. The double leather packing collars have longer wear-off period and extended service life.

Configuration of LC-31 High Performance Liquid Chromatograph
Standard configurations
•Standard P100 high pressure constant flow pump
•UV100 UV detector
•7725i manual injector
•Exformma Pronaos Series chromatographic column
•WS100 workstation software
The above configurations may vary upon users’ requirements.

Description:
•If the equipment performances and parameters required by the customer are beyond the scope of this introduction, please ask for it, we can introduce specifically.
•If the customer has other needs, please tell us, maybe we will give you a satisfactory answer.
•The Product may be modified at any time without notice.
•The final performance and parameters of the product are determined by the contract signed by the customer at the time of order.

Precision machining center in the workshop
High performance liquid chromatograph 7

Before you decide to purchase the instrument, we recommend that you come to visit the production workshop and take a look at the precision machining equipment of the instrument. Bring you direct access to the source of production, more real understanding of product performance and ex-factory price. "If the manufacturer is good, you will know when you look at the production site."